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Fig. 1. The loss of lin-35, dpl-1, efl-1 or
efl-2 function results in a reduced level of constitutive germ cell
apoptosis. (A) Time-course analysis of constitutive germ cell
apoptosis, which was analyzed in C. elegans hermaphrodites of the
indicated genotypes. Average numbers of apoptotic germ cells per gonad arm of
four independent experiments are shown. Error bars represent standard
deviations. For each experiment, a minimum of ten animals per genotype and
time point was scored double blind. All strains analyzed were homozygous for
the mutation ced-6(n2095). Except for the strain
efl-1(n3318), all strains were also homozygous for the
Plim-7ced-1::gfp integration bcIs39.
m+z- indicates that animals analyzed were
homozygous mutant progeny of heterozygous animals. The complete genotypes of
dpl-1(n3316) and efl-1(n3318) animals were
dpl-1(n3316) unc-4(e120);
ced-6(n2095); bcIs39 and
ced-6(n2095); efl-1(n3318), respectively.
(B) Analysis of constitutive germ cell apoptosis 40 hours post the L4
stage. Average numbers of apoptotic germ cells per gonad arm of two
independent experiments are shown. Error bars represent standard deviations.
For each experiment, a minimum of ten animals per genotype was scored blind.
All strains were homozygous for ced-6(n2095) and
bcIs39. Mutations of lin-35, dpl-1 and efl-2 used
were: lin-35(n745), dpl-1(n3643) and
efl-2(tm2359).
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