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Fig. 2. Quantification of Slit immunocytochemistry and the morphology of
aCC. (A) Cross-sectional and dorsal views of CNS at early (left)
and late (right) stages. The dimensions of the quantified volume (box) are:
width (W), 30.0 µm from the midline: depth (D), 5.3±0.4,
5.2±0.2 and 7.7±0.3 µm between the dorsal and ventral sides
of the midline glia cluster at hours 9, 14 and 17, respectively; and length
(L) 11.3±0.3 µm from the anterior to posterior ends of the midline
glia cluster at hour 9, and 4.5±0.2 and 4.7±0.1 µm from the
anterior to the posterior ends of the posterior commissure at hours 14 and 17.
(B) The z-stack images (1) are summed into a single 2D image
(2) and then consolidated into a single line (3) (see Materials and methods).
(C,D) DiI-labeled aCC (red) in the live wild-type embryo stained
with anti-HRP antibody (green, C), from which the tracing of the aCC is
obtained (D; see Fig. 7b
bottom). Scale bar: 5 µm.
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