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Figure 9


Fig. 9. Virtually wild-type neuropil but reduced Slit leads to small aCC dendrites in slit/+ and Sdc/Sdc mutants. (A,B) Detergent-free immunocytochemistry with anti-HRP (green) and anti-Slit (purple) in slit2/+ (A) and Sdc10608/Sdc10608 (B) embryos at hour 14. (C,D) Tracing s of slit2/+ (C) and Sdc10608/Sdc10608 (D) aCCs at hour 17. (E) Slit concentration topography in +/+ (n=12), slit2/+ (n=5), Sdc10608/Sdc10608 (n=12) and slit2/slit2 (n=3) at hour 14. Arrows and horizontal lines indicate the maximum numbers of dendritic tips per bin. (F) Histogram of the number of dendritic tips in +/+ (n=14), slit2/+ (n=12), Sdc10608/Sdc10608 (n=14) and slit2/slit2 (n=9) aCCs at hour 17. Arrows and horizontal lines indicate the neuropilar peaks. Scale bar: 5 µm.





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