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Fig. 1. Expression of Spalt4 in ectoderm and derived tissues.
Whole-mount in situ hybridization of (A) stage 3 chicken embryo,
(B) stage 5 chicken embryo (arrows indicate level of sections in D and
E), (C) stage 8 chicken embryo (arrow indicates levels of sections in
F). (D) Section through embryo in (B) at the level of the notochord.
(E) Section through embryo in B at the level of Hensen's node.
(F) Section through stage 8 embryo at midbrain level. (G)
Expression of Spalt4 in a stage 10 embryo sectioned at hindbrain
level. (H) Expression of Spalt4 in the otic pit (opi) of a
stage 13 embryo. Arrowhead points to the neural crest. (I) Expression
of Spalt4 in the otic vesicle (otv) of a stage 16 embryo. Arrowhead
indicates the neural crest. (J) Section through a stage 14 embryo at
eye level. (K) Section through a stage 17 embryo at forebrain level.
(L) Whole-mount in situ hybridization with Spalt4 in a stage
19 embryo showing strong Spalt4 expression in the forelimb. Arrow
shows the level of the section in M. (M) Section through the forelimb
in L. (N-Q) Comparison of stage 5 expression of (N). Spalt4
(O). Six1 (P). Eya2 and (Q) Sox3. ps, primitive
streak; hn, Hensen's node; no, notochord; otp, otic placode; opi, otic pit;
otv, otic vesicle; mb, midbrain; hb, hindbrain; re, retina; le, lens; fb,
forebrain; ol, olfactory epithelium; fl, forelimb; d, dorsal; v, ventral; aer,
apical ectodermal ridge.
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