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Figure 3


Fig. 3. Electroporation of a plasmid driving Spalt4 expression induces the expression of ectopic vesicles. (A) Embryo electroporated with a control GFP plasmid. (B) Embryo in A hybridized with Sox10 RNA probe. (C) Magnified view of embryo in A. (D) Sox10 in situ hybridization of control embryo in C. (E) Embryo electroporated with Spalt4-GFP construct. A number of ectopic vesicles are visible (arrows). (F) The ectopic vesicles in the Spalt4 electroporated embryo in E express Sox10 (arrows). (G) Embryo electroporated with Spalt4-GFP construct. A number of ectopic vesicles are visible (arrows). (H) The ectopic vesicles in the Spalt4 electroporated embryo in G express Notch1 (arrows). (I) Embryo electroporated with Spalt4-GFP construct. A number of ectopic vesicles are visible (arrows). (J) The ectopic vesicles in the Spalt4 electroporated embryo in (I) express EphA4 (arrows). OtV, otic vesicle; BA, branchial arch.





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