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Figure 6


Fig. 6. The effect of expression of Spalt4 in the trigeminal placode. Whole-mount in situ hybridization with a probe to NeuroD in embryos electroporated with (A) control plasmid or (B) plasmid overexpressing Spalt4. Arrows point to the approximate plane of section of trigeminal ganglia in C and D. (C,D) Sections at trigeminal ganglion level of double in situ hybridizations with Sox10 (light blue) and NeuroD (dark blue) in embryos electroporated with (C) control plasmid or (D) plasmid overexpressing Spalt4. (E,F) Sections through an embryo electroporated with Spalt4 on the right side only, and analyzed with GFP (E), or HuD (F) antibody. Scale bars: 100 µm.





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