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Figure 2


Fig. 2. Tead4 is expressed in preimplantation embryos and trophoblast cell lines. (A,B) Real-time RT-PCR was used to quantify the levels of Tead4 mRNA in unfertilized eggs, 1-cell embryos (1-C), 2-cell embryos (2-C), 8-cell embryos (8-C), morulae (M) and blastocysts (B) (A), as well as in trophoblast stem (TS) cells, trophoblast giant (TG) cells, embryonic stem (ES) cells and embryoid bodies (EB) (B). Each sample was internally normalized to Gapdh mRNA. Gapdh mRNA level per egg or embryo in A and Gapdh mRNA level per cell in B were essentially constant. Error bars represent the s.e.m. of three independent assays. (C,D) In situ hybridization was used to detect Tead4 transcripts at E6.5. Bright-field (C) and dark-field (D) images of an embryo in utero. Tead4 is broadly expressed in all extraembryonic layers and deciduum, but highest levels are in extra-embryonic (Ex) trophoblast cells of the ectoplacental cone (EPC, outlined by a dashed line). No expression was observed in the embryonic germ layers (Emb).





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