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Figure 5


Fig. 5. Tead4-/- embryos appear abnormal at E3.5 and fail to form blastocyst outgrowths in vitro. E3.5 embryos from heterozygous matings were collected and cultured in gelatin-treated tissue culture dishes. Tead4+/+ and Tead4+/- embryos appear as normal blastocysts with inner cell masses (icm), trophectoderm (te), blastocoel cavities (bc) and zonae pellucidae (zp), whereas Tead4-/- embryos appear as abnormal morulae. After 5 days in culture, the embryos and their outgrowths (tg, trophoblast giant cells) were collected for genotyping.





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