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Fig. 2. Identification of the MAB4/ENP gene. (A)
Map-based cloning. The MAB4/ENP locus was mapped to lie
between the F3L17 and F10M6 markers on chromosome 4. The number of
recombinants are indicated under these markers (recombinant
chromosomes/analyzed chromosomes). The mab4-1 mutation is a deletion
of 9 bp in At4g31820, which loses three amino acids, G,L and Y. The
enp mutation is a point mutation, C to T, which creates a stop codon.
In mab4-2 mutants, T-DNA is inserted at the 5' untranslated
region of the gene. ARE, auxin-responsive element; WUS, WUS-binding site.
(B) The deduced amino acid sequence of MAB4/ENP, shown in comparison
with the sequence of NPH3 and RPT2. Asterisks denote residues deleted in the
mab4-1 mutant. Black circle indicates the mutated residue in the
enp mutant. Gray and black shadings indicate residues identical in
the two and three sequences, respectively. (C) Phylogenetic tree of
Arabidopsis MAB4/ENP homologs and the two related rice homologs and
CPT1. The tree was obtained by the neighbor-joining method using 1000
bootstrap replicates, generated with CLUSTALW. Black circles indicate rice
NPH3-like protein.
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