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Fig. 6. TCL1 suppresses the expression of GL1. (A) RT-PCR
analysis of TTG1, GL1, GL2 and GL3 transcripts in
Arabidopsis seedlings overexpressing HA-TCL1. Expression of
ACTIN2 provided a control. (B) Suppression of
GL1::GUS reporter by TCL1. Shown are the expression of
GL1::GUS reporter in wild-type (Col) and in 35S:HA-TCL1
backgrounds. In the GL1::GUS reporter, the expression of GUS
was driven by a chimerical genomic sequence consisting of the immediate
5' upstream sequence, the first intron, and the 3'-UTR region of
GL1 (Wang et al.,
2004). (C) RT-PCR analysis of TCL1 transcript in
various tissues/organs. Expression of ACTIN2 provided a control.
(D) RT-PCR analysis of GL1 and GL2 transcripts in the
developing inflorescence of tcl1-1 mutants. Expression of
ACTIN2 provided a control.
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