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Figure 5


Fig. 5. Expression pattern of ApoER2 transcripts at different developmental time points. (A-F) In situ hybridization for ApoER2 on sagittal sections of E16.5 (A), E18.5 (C) and P7 (E) wild-type cortex showed expression throughout the whole cortical wall. Higher magnification of the neocortex revealed the strongest signal in upper cortical layers (B,D,F). (G-I) Double fluorescence in situ hybridization for Cux2 (G, green) and ApoER2 (H, red) shows colocalization (I, yellow) of the two markers, indicating that late generated cortical neurons express ApoER2. Scale bars: 200 µm in A,C,F-I; 100 µm in B,D; 500 µm in E.





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