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Fig. 1. Spermatogonial transplantation into testicular seminiferous tubules of XX/Sry and XY W/W^v (control) males. (A,B) HE staining of the intact XX/Sry and XY W/W^v testes at 8 weeks of age, showing lack of germ cells in most tubules of both testes. (C-J) The recipient testes were injected with spermatogonial-cell suspension prepared from immature ROSA26 (C-F), wild-type (G,H) and Sl/Sl^d (I,J) testes, and were then histologically examined at 3 months after transplantation. (C-F) LacZ staining of the transplanted testes, visualizing ROSA26-derived XY germ cells in XX/Sry (C,E) and XY W/W^v (D,F) testes. Asterisks indicate LacZ-negative Sertoli cells located at the basal region of the seminiferous tubules. Insets show higher-magnification images of boxed area. (G-J) HE staining of XX/Sry (G,I) and XY W/W^v (H,J) testes injected with the wild-type-derived (G,H) or Sl/Sl^d-derived (I,J) testicular cell suspension. Higher-magnification images of recipient tubules with donor germ cells are shown on the right. Scale bars: 50 µm in A,G,I and inset of E; 200 µm in E.

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