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Figure 1


Fig. 1. NAD(P)H and FAD++ autofluorescence originate from both mitochondrial and cytosolic compartments in mouse oocytes. Schematic representation of the metabolic pathways producing NADH and NADPH in the cytosol and the mitochondria of a eukaryotic cell. The possible sources of FAD++ fluorescence in the mouse oocyte are highlighted in green and are the pyruvate dehydrogenase (PDH) complex and the complex I of the mitochondrial electron transport chain. See main text for details. {alpha}-KG, alpha-ketoglutarate; Ac-CoA, acetyl coenzyme A; ANT, adenine nucleotide translocase; CiC, citrate carrier; LDH, lactate dehydrogenase; ME, malic enzyme; MDH, malate dehydrogenase; MCT, mono carboxylate transporter; NAD(P)+, oxidised nicotinamide adenine dinucleotide (phosphate); NADH, reduced nicotinamide adenine dinucleotide, NADP-ICDH, NADP-dependent isocitrate dehydrogenase; NK, NAD kinase; PentosePP, pentose phosphate pathway, TrH, transhydrogenase.





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