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Figure 8


Fig. 8. Regulation of early development by the redox potential. (A) Effect of high pyruvate (1 mmol/l) on early development of mouse embryo. The bars indicate the percentage of embryos at each stage of development observed after 5 days of culture in control conditions (black bars), in H-KSOM/AA containing 1 mmol/l pyruvate (white bars) and in H-KSOM/AA containing 1 mmol/l pyruvate and 8 mmol/l TEMPO (stippled bars). Asterisk a (*a) indicates that this value is significantly different from the control value. Asterisk b (*b) indicates that this value is significantly different from the value of the white bar (high pyruvate treatment). (B) Effect of oxamate on early development of mouse embryos. Black bars: control condition. White bars: culture in the presence of 10 mmol/l oxamate. Asterisk indicates that this value is significantly different from the control value. (C) Effect of oxalomalate on early development of mouse embryos. Black bars: control condition. White bars: culture in the presence of 9 mmol/l oxalomalate. Asterisk indicates that this value is significantly different from the control value. P=0.05.





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