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Fig. 7. Wnt/ß-catenin signaling regulates FGF signaling during fin
regeneration. (A) fgf20a expression as detected by
semi-quantitative RT-PCR is greatly reduced in Dkk1-overexpressing fins at 3
hpa. Wild-type and hsDkk1GFP transgenic fish were treated according to the
schematic (hs, heat shock; amp, amputation; the green line indicates
inhibition of Wnt/ß-catenin signaling), and RNA was harvested from the
tissue adjacent to the amputation plane of nine wild-type and two groups of
nine hsDkk1GFP transgenic fins. odc1 amplification serves as a
loading control. The experiment was repeated four times using two sets of
biological samples and representative results are shown. (B)
fgf20a expression is greatly reduced as detected by in situ
hybridization in hsDkk1GFP transgenic fins (5 of 6 fins) at 24 hpa as compared
with wild-type fins. (C) sprouty4 expression is greatly
reduced 6 hours after heat shock in hsDkk1GFP fins (n=3) at 72
hpa.
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