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Fig. 2. Overexpression of N-cadherin inhibits NC delamination.
(A,C,E) Electroporation of control-GFP. (A) Segmentally
migrating labeled NC (arrows) in whole-mounts. (C) Early delaminating NC
(arrows) opposite a dissociating somite (S) in transverse section. (E)
GFP-labeled mesenchymal NC cells emigrate from NT explants along with
unlabeled progenitors evident with phase contrast optics.
(B,D,F) Electroporation of full-length N-cadherin. No NC
delamination occurs in whole-mounts (B), in sections opposite an already
dissociated somite (D), or from NT explants (F). Note that unlabeled cells
emigrate normally, yet transfected cells round up as in vivo, with no evidence
of cell death (F and data not shown). (G,H) Control and
N-cadherin-treated embryos, respectively, fixed 35 hours after transfection
when peripheral ganglia are already coalesced. (G) Note the presence of GFP+
cells (arrows, green) that colonize the HNK-1+ DRG and SG (red). (H) By
contrast, N-cadherin+ cells remain in the dorsal NT where they express ectopic
HNK-1 (arrow). n=6 for each treatment. Abbreviations: DM,
dermomyotome; DRG, dorsal root ganglion; NT, neural tube; S, somite; scl,
sclerotome; SG, sympathetic ganglion. Scale bar: 28 µm for C,D; 55 µm
for E,F; 25 µm for G,H.
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