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Fig. 5. The normal downregulation of N-cadherin protein along the axis is
regulated by BMP4. (A-D) Treatment with BMP4-coated beads
(* in B,C; n=7) or electroporation with BMP4-DNA/GFP
(arrowhead in D; insert depicts localization of transfected BMP/GFP;
n=8) for 8 hours downregulates N-cadherin immunoreactivity at levels
of rostral and caudal segmental plates. At these levels, N-cadherin is still
expressed in the dorsal NT under normal conditions (A) and in matching
controls (not shown). (E,F) Sections through a dissociating
somite level 15 hours after electroporation of control-GFP (E; n=8)
and noggin-GFP (F; n=9). (E) Note the absence of N-cadherin in the
dorsal NT from which N-cadherin-negative NC cells emigrate (arrows depict
phase-bright NC in main image and GFP+ NC in insert). (F) By contrast, noggin
overexpression (green cells in insert and surrounding area) maintains
N-cadherin and no NC emigration is apparent. Similar results were obtained by
staining with anti-N-cadherin antibodies directed to either the extracellular
or intracellular domains of the protein; the data presented stem from the
latter. Abbreviations: CSP, caudal segmental plate; RSP, rostral segmental
plate; scl, sclerotome. Scale bar: 25 µm.
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