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Figure 1


Fig. 1. Nkx2.2 derivatives are active in pancreatic cell lines. (A) Schematic of Nkx2.2-dominant-derivative transgenes. (B) The Nkx2.2-dependent luciferase reporter (pFoxLuc1-7xNk2prl) was co-transfected with pcDNA3 alone (light grey), pcDNA3-2.2hdVP16 (dark grey) or pcDNA3-2.2hdEnR (white) expression plasmids in ßTC3- or PANC cells, and is activated with Nkx2.2hdVP16. Error bars represent the standard error (s.e.m.) of three independent experiments. 2.2HD, Nkx2.2 homeodomain; myc, myc epitope tag; VP16, VP16 activation domain from herpes simplex virus; EnR, Engrailed repressor domain.





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