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Figure 3


Fig. 3. The Nkx2.2-repressor transgene partially rescues the Nkx2.2-null pancreas phenotype. (A-J) Immunofluorescence staining for insulin (red), glucagon (blue) and amylase (green) (A,C,E,G,I), or ghrelin (red; B,D,F,H,J) on E18.5 pancreata for wild-type (A,B), Nkx2.2-null (C,D), Nkx2.2-/-;Nkx2.2hdEnR (Line 7414; E,F), Nkx2.2-/-;Nkx2.2hdVP16 (Line 7318; G,H) and Nkx2.2-/-; Nkx2.2hdCon (Line 5635; I,J) embryos (20x magnification) demonstrates that the Nkx2.2-repressor transgene can restore glucagon- and insulin-positive cells. (K-M) Quantitative PCR for glucagon (K), insulin (L) and ghrelin (M) on E16.5 pancreatic RNA isolated from wild-type (black bars), Nkx2.2-/- (dark grey bars) and Nkx2.2-/-; Nkx2.2hdEnR (light grey bars) embryos. (N) Summary of cell counting data demonstrates that insulin-positive cell numbers are restored to approximately 20% of wild-type levels and glucagon-positive cell numbers are restored to wild-type levels (E18.5, n=3).





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