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Fig. 7. Nkx2.2 interacts with Grg3 in the embryonic pancreas. (A-D)
RNA in situ hybridization for Grg3 at E12.5 (A) and E16.5 (C,E,F),
and for Nkx2.2 at E12.5 (B) and E16.5 (D). Adjacent sections
demonstrate similar expression patterns throughout pancreas development. In C
and D, outlines represent selected overlapping expression areas.
(E,F) Combined RNA in situ hybridization and
immunohistochemistry on E16.5 pancreata demonstrate overlapping expressions
between Grg3 (blue) and glucagon (brown; arrows in E), and distinct
expression domains between Grg3 (blue) and amylase (brown; F).
(G) Co-immunoprecipitation for Nkx2.2 and Grg3: PANC cell lysates were
incubated with Grg3 antibody and were western blotted with antibodies against
Nkx2.2. Lanes 1 and 4: vector-only control; lanes 2 and 5: Nkx2.2 transfected
with Grg3; lanes 3 and 6: Nkx2.2
TN transfected with Grg3. Lanes 1-3 are
input samples and lanes 4-6 are Grg3 pull-down samples. Nkx2.2 is
co-immunoprecipitated with Grg3 (lane 5).
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