spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Fate of Notch1-activated cells in the endocardial lineages. (A,B) Labeling of the heart (E10.5) in whole-mount (A) and after dissection (B). Notch1 activity results in lacZ labeling within the outflow tract (OFT, arrow) and the ventricles (not shown). (C,D) Exclusive endocardial staining of the heart, the outflow tract and the lining of the valves (arrowhead) at E14.5. Myocardial cells are not stained. BA, branchiocephalic artery. (E,F) N1::cre-labeled endocardial cells continuously receive a Notch1 signal (arrowheads) at E14.5, as shown by {alpha}-VLLS staining. (G,H) In the adult heart, most endocardial cells retain label; the endothelial lining of the valve and the arteries (H, arrows), where Notch1 is required, are also labeled. (I,J) Comparison of the complementary staining patterns of endocardial cells (arrowhead) and cardiomyocytes in (I) N1::cre and (J) N1-/-;N1+/+;R26 lacZ chimeric hearts, respectively. Note that Notch1 is not active in cardiomyocyte cells(I) and also not required(J). Magnification: A, 16x; B, 50x; C,G,10x; E,F,40x; I,J 63x.





Right arrow Return to article