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Fig. 7. Pax6 overexpression does not alter the expression profile of
cortical regionalization markers. (A,B) Immunohistochemistry
on sagittal sections through the cortex of (A) wild-type and (B)
PAX77+ E12.5 embryos showing the expression of Emx2. The
gradient of Emx2 expression is conserved in the cortex of PAX77 mice and
overall levels are not reduced. (C,D) Immunohistochemistry on
horizontal sections through the cortex of (C) wild-type and (D)
PAX77+ E16.5 embryos showing the expression of Ephrin B2
in the right hemisphere. In the wild-type embryo, Ephrin B2 is strongly
expressed in a caudal domain (arrowhead shows the anterior limit of the Ephrin
B2 caudal expression domain). This caudal domain is present and does not
appear contracted in the PAX77 cortex. (E,F) In situ
hybridizations on sagittal sections through the forebrain of (E) wild-type and
(F) PAX77+/+ E18.5 embryos showing the expression of
Id2 mRNA. In the wild-type embryo, Id2 is strongly expressed
in layers 2 and 3 in an anterior domain (black arrow shows the posterior limit
of this domain), and in layer 5 in a caudal domain (white arrowhead shows the
anterior limit of this domain). The expression of Id2 appears
unaltered in the PAX77 cortex. Scale bars: 200 µm in A-C; 0.5 mm in
D-H.
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