|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. Identification of a candidate svs mutation. (A)
Representative Southern blot of genomic DNA from svs, C57BL/6By (B6), and
Balb/cBy (Balb) mice digested with BamHI and probed with a fragment
from Fgfr2. Note the shift of a major band only in svs mutant mice
(arrowhead). (B) PCR of svs, C57BL/6By (B6), and Balb/cBy (Balb) mouse
genomic DNA using primers flanking the candidate mutation. The parental
control strains Balb/cBy and C57BL/6By have a 399 bp band, whereas svs mutant
mice have an 890 bp band. (C) Genomic structure of Fgfr2. The
horizontal arrow indicates the direction of transcription; the vertical lines
indicate exons; and the vertical arrow indicates the intron which harbors the
svs insertion (top). Sequencing of PCR products from svs mutant mice revealed
a 491 bp insertion flanked by intronic Fgfr2 sequences (bottom).
Abbreviations: mChr7, mouse chromosome 7.
|
