spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. In S2 cells, Abl promotes actin accumulation while Cta promotes myosin accumulation. S2 cells are shown, and the antigens, transfections and RNAi treatments are indicated. (A) UAS-Abl::GFP fusion construct. (B-E''') Transfected cells. Arrows indicate UAS-Abl overexpressing cells in B,C; UAS-activated Cta in D; and UAS-activated Rho in E. Arrowheads indicate untransfected controls. Abl-transfected cells display elevated phosphorylated Tyr (P-Tyr; B') and concentrated actin, but show no change in Myo-P (P-Myo) expression (C). (D) Cta-transfected cells display concentrated P-Myo, but not actin. (E) Rho-transfected cells exhibit concentrated actin and P-Myo, which are organized into a central ring. (F-H') abl RNAi does not block Rho gain-of-function. (F) Control RNAi. (G,G') Control RNAi+active Rho. Notice increased Rho and concentrated actin relative to F,F'. (H,H') abl RNAi+active Rho. Rho and actin localization is similar to active Rho alone (G,G'). (I) Immunoblot showing knockdown of the Abl protein from the S2 cells shown in G-H'. Samples were non-adjacent on the same gel. Pnut, loading control; PTyr, Phospho-Tyrosine. Scale bar: 30 µm.





Right arrow Return to article