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Figure 6


Fig. 6. Actin localization is altered in abl and RhoGEF2, but not in cta, mutants. Embryos and antigens are indicated. (A-D) Wild type; (E-H) abl mutant; (I-L) cta mutant; (M-P) RhoGEF2 mutant. (A,E,I,M) Late cellularization. Actin is localized basally in all genotypes (arrows), but also accumulates apically (arrowheads) in abl (E) and RhoGEF2 (M) mutants. Ena is diffusely localized in wild-type (A'), and in cta (I') and RhoGEF2 (M') mutants, but accumulates apically in abl mutants (E', arrowheads). (B,F,J,N) VF initiation. Ectopic apicolateral actin localization (arrowheads) occurs in abl (F) and RhoGEF2 (N) mutants, but not in wild-type (B) or cta mutants (J). (F) Red arrowhead shows accumulated actin in constricting cells. (C,G,K,O) Close-up of the VF. (C) Wild-type. Actin is at apical AJs in most cells (white arrowhead). In the mesoderm, actin accumulates apically in constricting cells (yellow arrowhead), and disappears basally (arrow). (G) abl mutant. Ectopic actin in apicallyconstricting cells (yellow arrowhead) and non-constricting cells (white arrowhead). Arrow indicates the disappearance of normal basal actin. (K) cta mutant. Actin localization is normal (arrow, arrowheads as in C). (O) RhoGEF2 mutant. Actin defects resemble those in abl mutants. Arrows and arrowheads are as in G. (D,H,L,P) Close-up of the VF. Arrows indicate non-mesodermal Ena. (D) Wild type. Ena concentrates at AJs in non-mesodermal cells, but remains diffuse in mesodermal cells (arrowhead). (H) abl mutant. Ena accumulates apicolaterally in non-mesodermal (arrow) and mesodermal (arrowhead) cells. (L,P) Neither cta (L) nor RhoGEF2 (P) mutants accumulate ectopic mesodermal Ena (arrowhead). (Q-R'') RhoGEF2 mutant at late gastrulation. In non-mesodermal cells, excess apical actin colocalizes with Ena in ectopic structures (arrows). (R) Close-up of Q. (S,T) ena heterozygosity suppresses the VF phenotype of abl mutants. (S) abl mutant. (T) ena/+;abl embryo. Scale bar: 20 µm.





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