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Fig. 7. Fz3 and Fz6 localization in mouse inner ear sensory epithelium.
(A) Immunolocalization of Fz3 (green) in the organ of Corti at P0.
Actin bundles are stained with phalloidin (red). IHC, inner hair cells; OHC1,
inner row of outer HCs; OHC2, central row of outer HCs; OHC3, outer row of
OHCs. (B) In the vestibular system, Fz6 (green) is expressed in
supporting cells and sensory neurons. In the Fz3-/-
crista, sensory hair bundle kinocilia orient to the right, as indicated by the
hole on the actin-rich cuticular plate (phalloidin, red), which covers much of
the apical face of the sensory neurons. Fz6 localizes preferentially on
lateral faces that orient perpendicular to the axis of the sensory hair
bundles. (C-E) WT:Fz6-/- mouse embryo chimeras and
Fz6 protein localization. (C,D) Organ of Corti from a
WT:Fz6-/- embryo chimera immunostained with anti-Fz6 (red)
and anti-ß-galactosidase (green) to visualize nuclear
ß-galactosidase encoded by Fz6-/- (lacZ
knock-in) cells (asterisks). Phalloidin (blue) stains actin bundles.
ß-galactosidase expression varies among Fz6-/- cells.
The nuclei (D) are present in a deeper plane than the apical edge of the
cells, where Fz6 accumulates (A). (E) An enlargement of the right corner of C,
showing Fz6 punctate intracellular accumulation only in WT cells. Reproduced
with permission from Wang et al. (Wang et
al., 2006b).
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