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Fig. 7. Accumulation of FM4-64 in the intestine after injection into the
pseudocoelom (basolateral delivery). (A) FM4-64 fluorescence in
sel-2(+) and sel-2(ar219). (B)
FM4-64 fluorescence (middle panel, red) is shown relative to autofluorescent
granules (left panel, green); co-localized signals are shown in yellow in the
merge (right panel). Arrows indicate irregularly shaped, FM4-64 labelled
compartments that are not coincident with autofluorescence in
sel-2(ar219). (C) Quantification of the
`sel-2' mutant phenotype (see text). Rescue of the phenotype with an
extrachromosomal array carrying the sel-2 genomic region and
phenocopy with sel-2(RNAi) is also shown. All scoring was
done blind to genotype. Each bar is the mean of at least two independent
experiments, and error bars are the s.e.m. The total number of worms scored is
indicated on each bar.
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