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Fig. 2. Disruption of Fgfr2 alleles leads to perturbed prostate morphogenesis. (A) The urogenital sinuses were dissected from embryos or postnatal pups carrying ROSA26/NKX3.1-Cre, and Fgfr2^flox or wild-type Fgfr2 alleles at the indicated days. The tissues were lightly fixed and stained with X-Gal, as described. The stained tissues representing each prostatic lobe are indicated. Notice no significant difference exists in prostatic buds at E17.5 between Fgfr2^cn and wild-type controls. Insert: section from the same tissue showing that NKX3.1-Cre efficiently excised the silencing cassette of the ROSA26 allele. (B) The prostate and urethra were dissected from mice at the indicated ages (left panels). Right panels: dorsolateral prostate lobes dissected from tissues shown in left panels. (B',B'') Epithelial ducts were dissected from dorsolateral prostates of 6-week-old mice with the indicated genotypes. (C) The prostate tissues were collected from Fgfr2^cn and control mice at the indicated ages, and proliferating cells were identified immunohistochemically by expression of PCNA. Inserts: high-magnification views from the same sections. ap, anterior prostate; dlp, dorsolateral prostate; vp, ventral prostate; u, urethra; b, bladder; s, seminal vesicle; F/F, homozygous Fgfr2^flox mice; CN, Fgfr2^cn mice. Scar bars: 2 mm.

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