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Figure 3


Fig. 3. Development of ectopic cephalic outgrowths after SmedroboA RNAi. Living planarians imaged at 16 days (A) and 33 days (B) after amputation. (C-E) Cephalic neural markers in outgrowths. (C) Visual-cell marker, VC-1; confocal projection at 35 days after amputation. (D) Sensory-cell marker, cintillo (Oviedo et al., 2003); arrow indicates photoreceptor pigment cells. (E) Clone H.10.2f, marker of cephalic ganglia. (D,E) At 16 days after amputation. In A-E, arrowheads indicate ectopic outgrowths. (F) Ectopic Smed-netrin1 expression (arrowheads) near an ectopic pharynx (red asterisk); 18-day regenerate. (G-L) Expression of the ventral marker anosmin-1 (AY066061) in an 18-day regenerate. (G) Control: anosmin-1-positive cells are not observed in dorsal views. (H) Smed-roboA RNAi: anosmin-1-positive cells are detected dorsally. (I-L) Control (I,J) and Smed-roboA RNAi (K,L) planarians viewed ventrally (I,K) and dorsally (J,L). Anterior to the left. Scale bars: 1.5 mm in A (bar shown in B); 1 mm in B; 100 µm in C-E; 100 µm in F; 500 µm in I-L.





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