DEV02780 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Nkx2.1b expansion is not at the expense of the Dlx-expressing subpallium. Double labelling for Dlx2 (red/fluorescence) and Nkx2.1b (purple) at 36 hpf reveals that the expansion of Nkx2.1b domain in cavefish is not at the expense of the Dlx-only expressing subpallium, but rather due to an antero-ventral expansion. Dotted lines delineate the relative expression domains in the telencephalon, on high power micrographs. p, pineal gland.

• Supplemental Figure 2 -

  Fig. S2. proliferation and size of the basal diencephalon is Shh-dependent. Cavefish embryos were treated with 20 μM cyclopamine from 15-24 hpf and allowed to develop until 48 hpf. A-B, C-D, and E-F show transverse sections through the preoptic region (po), the posterior hypothalamus (hyp) and the optic tectum (ot), respectively, of treated embryos where proliferation is assessed with anti-phosphohistone H3 immunostaining. Scale bar: 50 μm for all panels.

• Supplemental Figure 3 -

  Fig. S3. Size reduction in the basal diencephalon of cavefish embryos after cyclopamine treatment. Nomarski pictures of transverse sections through the anterior (A-C) and and posterior (D-F) basal diencephalon of cavefish embryos treated with the indicated concentration of cyclopamine (also see Fig. 3). The dotted lines delineate the limits of the basal diencephalon, bordered laterally by the eyes and dorsally by a commissure at the level of the hypothalamus. Arrows indicate the dose-dependent reduction in the width of the diencephalon.