DEV02790 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Amino acid sequence comparison of wild-type and mutant forms of CATP-1. (A) Comparison of C. briggsae and C. elegans CATP-1. (B) Mos1 insertion in catp-1 introduces a late STOP that truncates the last three transmembrane regions of CATP-1. catp-1 short isoform generates a truncated protein.

• Supplemental Figure 2 -

  Fig. S2. Amino acid sequence comparison of the putative transmembrane domains M1-M8 bearing cation specificity between H⁺/K⁺- and Na⁺/K⁺-ATPases. Although global organization is conserved among type IIC ATPases, no subclass-specific amino acid signature can be detected in CATP-1.

• Supplemental Figure 3 -

  Fig. S3. catp-1(kr17) does not suppress lifespan elongation of a daf-2(m41) mutant. Life-span measurement was performed at 25°C (n>40 individuals). Mean life spans were: wild type, 17.6; catp-1(kr17), 14.5; daf-2(m41), 34.2; catp-1(kr17); daf-2(m41), 34.9 days after L4.