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Figure 2


Fig. 2. Cks30A, Cort and Fzy regulate overall cyclin levels in the egg. Western blots of eggs aged 0-2 hours from unfertilized (females crossed to X0 males) wild type (yw), Cks30AKO/Cks30AKO (cks), cortQW55/cortRH65 (cort), fzy6/fzy7 (fzy), and cortQW55,fzy6/cortRH65,fzy7 (cort,fzy) kept at 29°C and probed for cyclin B3, cyclin A and cyclin B. Cdk1, a stable cell-cycle regulator, serves as a loading control. Cyclin B is slightly elevated in Cks30A, more so in fzy and cort, and is highly elevated in fzy; cort double mutants. Cyclin A and cyclin B3 are both elevated in cort and fzy, but elevation is higher in cks30A and fzy; cort double mutants.





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