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Fig. 7. Ectopic expression of the Meox1-cre/R26 reporter gene in
cardiac outflow tract and renal cortex. (A) Real-time RT-PCR of
Meox1 mRNA expression at E9.5-E18.5 showed that Meox1
expression peaked during somitogenesis at E9.5-E10.5 and thereafter declined
rapidly. (B-E) X-gal staining of cryosections (B-D) and whole-mount
preparations of organs (E) from P2 mice confirmed reporter gene expression in
somite-derived tissues, including dermis, vertebrae, skeletal muscle and
microvessels. Arrows in B indicate dermal papilla and arrowheads indicate
epidermal follicular epithelium. Thin arrow in C indicates chondrocytes, thick
arrow indicates ossification centre in ventral part of vertebrae, arrowhead
indicates an intervertebral disc that derive from the notochord. Arrows in D
indicate the external oblique muscle surrounded by white adipose tissue.
Arrowheads in E indicate blood vessels that spread across the surface of the
unlabelled uterus. (F-I) Ectopic expression was detected in the
arterial pole of the heart: whole-mount staining showed expression in
ascending aorta (white arrow in F) and aortic arch, but also in patches of
atrial myocardium (black arrow in F). Paraffin sections of whole-mount stained
hearts confirmed expression in SMCs in proximal aorta (ao) and pulmonary trunk
(pt) (G). Expression was also detected in a fine caliber branched network
(arrowheads in H) on the heart surface that seemed to be distinct from blood
vessels (arrows in H). Double staining against lacZ and Acta2 on
paraffin sections confirmed that the network on the heart surface (arrowheads
in I) were distinct from the coronary arteries (arrows in I). (J-M)
Ectopic expression in kidney: en bloc X-gal staining of kidney slices revealed
reporter expression in the cortex (white asterisk in J) but not in the pelvic
region (black asterisk). Detailed inspection of the cortex in paraffin
sections revealed that collection ducts (arrows in K) lacked expression,
whereas other components were homogenously stained (asterisk indicates
s-shaped nephron anlagen). Other derivates of intermediate mesoderm such as
adrenal cortex (asterisk in L) and gonads (white arrow in M) lacked staining.
Arrows in L indicate reporter-expressing cells in the adrenal capsule,
arrowheads in M indicate blood vessels; ed, epidermis; de, dermis.
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