|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Schematic of experimental strategy. (A) Xenopus
embryos were injected into both animal blastomeres at the two-cell stage with
Gata5 (500 pg) mRNA. Ectodermal explants were isolated at stage 9
from both Gata5-injected and uninjected embryos and cultured until
stage 28 (tailbud). Transcriptional differences were analyzed on 5000-clone
gastrula-stage cDNA microarray
(Munoz-Sanjuan et al., 2002).
An aliquot of each RNA sample was assayed for expression of the indicated
Gata5 targets by RT-PCR. ODC was used as loading control. (B)
Pie chart of the classification of the genes upregulated by Gata5
based on the GO molecular function categories. The majority of the upregulated
clones in the Gata5 array fall into four main categories: (1)
catalytic activity (26%); (2) binding activity (26%), including the large
group of nucleic-acid-binding as well as protein-binding factors; (3)
hypothetical/unknown function (13%), including full-length sequences conserved
in the mouse and human databases the function of which is unknown; (4) no
database match (13%), including a number of clones with no hits in database
searches, which might be either genes unique to the frog or partial cDNAs.
Edd, endodermin; -RT, minus reverse transcriptase; ODC, ornithine
decarboxylase.
|
