DEV015248 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Changes in calretinin (CR) expression in Runx3^−/− DRG during development. Photomicrographs showing CR immunoreactivity at E14.5 (A,D), E18.5 (B,E) and P0 (C,F) in wild-type (A-C) and Runx3^−/− (D-F) DRG at the level of Th11. (G) Quantification of CR⁺ neurons in DRG from E13.5 to P0. The number of CR⁺ DRG neurons in Runx3^−/− (black triangles) decreased at E15.5 and E18.5 as compared with the wild type (white triangles). Data are shown as mean ±s.e.m.; *P<0.05, **P<0.01. Scale bar: 100 µm.

• Supplemental Figure S2 -

  Fig. S2. Photomicrographs showing DiI-labeled afferents in the spinal cord of Runx3^+/+; Bax^−/− and Runx3^−/−; Bax^−/− mice at the thoracic level. DiI-labeled afferents project to the dorsal horn, the intermediate zone and the ventral horn in Runx3^+/+; Bax^−/− mice (A), but not to the ventral horn in Runx3^−/−; Bax^−/− mice (B) at P0. Scale bar: 100 µm.

• Supplemental Figure S3 -

  Fig. S3. Coexpression of TrkC and cutaneous markers in wild-type and Runx3^−/− DRG. (A,B) Double staining of Cgrp and TrkC in wild-type (A) and Runx3^−/− (B) mice Th5 DRG at P0. (C,D) Double staining of TrkB and TrkC in wild-type (C) and Runx3^−/− (D) Th5 DRG at P0. TrkC⁺ neurons coexpressed Cgrp or TrkB both in the wild-type and Runx3^−/− DRG. Scale bar: 100 µm.

• Supplemental Figure S4 -

  Fig. S4. Coexpression of CR, Runx3 and TrkC. Triple staining of CR (A), Runx3 (B) and TrkC (C) in wild-type Th9 DRG at P0. Coexpression of CR and Runx3 (D), CR and TrkC (E), and Runx3 and TrkC (F). (G) Merged image. CR⁺ neurons coexpressed TrkC but not Runx3. Scale bar: 100 µm.