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Files in this Data Supplement:
Fig. S1. Labeling of early and late born cortical neurons in reeler mice, Dab1−/−mice and ApoER2/Vldlr double mutants at P7 using ER81 and Cux2. In situ hybridization for ER81 (A,C,E), a marker of early generated neurons, showed a tendency for mainly superficial layers to be stained in sagittal brain sections of these mutants, whereas in situ hybridization for Cux2 (B,D,F), a marker of late generated neurons, showed a reverse gradient. Scale bar: 200 μm.
Fig. S2. Labeling of interneurons in the cortex of wild-type animals, ApoER2 mutants and Vldlr mutants. Immunostaining for calretinin mainly labeled neurons in superficial layers in wild-type animals (A) and in ApoER2 mutants (B). In ApoER2 mutants, some labeled cells were in addition found in deep cortical layers (B). No obvious differences between wild-type animals and ApoER2 mutants were noted in sections immunostained for parvalbumin (C,D). In Vldlr mutants, a few calretinin-positive cells were observed in the marginal zone (I), which appeared cell-free in wild-type animals (E). No clear differences were observed with the interneuron markers parvalbumin, GAD67 and with reelin (F-H,J-L). Scale bars: A-D, 200 μm; E-L, 50 μm.
Fig. S3. Layer formation in the olfactory bulb is altered in ApoER2 mutants. Immunostaining for calbindin on frontal sections through the olfactory bulb of adult wild-type mice showed strong expression in the glomerular cell layer (A). A similar staining pattern was observed in Vldlr−/− mice (B). By contrast, in ApoER2 mutants (C), two separate calbindin-positive cell layers were found, a superficial one in typical periglomerular position, and a deep one in the external plexiform layer close to the reelin-containing mitral cell layer. Immunostaining for BLBP on frontal sections through the olfactory bulb at E16.5 showed no obvious differences in the radial glial scaffold between wild-type (D) and ApoER2 mutant (E) mice. Glom CL, glomerular cell layer; EPL, external plexiform layer; MCL, mitral cell layer; GCL, granule cell layer. Scale bars: A-C, 50 μm; D,E, 100 μm.
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