DEV014993 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Phylogenetic tree and sequence alignment of OBE homologue. (A) Phylogenetic tree using complete sequences constructed with ClustalX2.0. The tree was made with 1000 bootstrap trials, with correction for gaps in sequences. PsOBE1, NbOBE1 and OsOBE1 are putative orthologs of OBE1 from Pisum sativum, Nicotiana benthamiana and Oryza Sativa, respectively. (B) Alignment of the PHD finger domains of the proteins described in A with the following known PHD finger proteins: MALE STERILITY 1 (MS1) (Wilson et al., 2001), MALE MEIOCYTE DEATH 1 (MMD1) (Yang et al., 2003), EARLY BOLTING IN SHORT DAYS (EBS) (Pineiro et al., 2003) and VERNALIZATION-INSENSITIVE 3 (VIN3) (Sung and Amasino, 2004).

• Supplemental Figure S2 -

  Fig. S2. Characterization of loss-of-function mutants of OBE1 and OBE2. Semi-quantitative RT-PCR analysis of OBE1 and OBE2 expression in wild-type, obe1-1 and obe2-1 plants. TUB expression was used as a control.