DEV015842 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Interactions between 35S::LFY-FLAG and UFO. (A) 35S::LFY-FLAG plants display a phenotype identical to that of 35S::LFY plants (Weigel and Nilsson, 1995), indicating that the 35S::LFY-FLAG transgene retains full LFY function. 35S::LFY-FLAG plants produce solitary flowers in the axils of rosette leaves, as well as terminal flowers on the primary stem. Lateral flowers are formed but internode elongation is suppressed. (B,F,G) 35S::LFY-FLAG; ufo-2 plants have flowers that show an identical phenotype to ufo-2 but show a precocious floral transition characteristic of 35S::LFY-FLAG plants (B). This phenotype is apparent in the terminal flowers and short internodes (F) and the characteristic ufo-2-like phenotype is shown in G. (C-E) 35S::LFY-FLAG;35S::UFO-myc; ufo-2 plants used for co-immunoprecipitation. The double transgenic plants in the ufo-2 background survive to produce inflorescences unlike those in a wild-type background (C). Unlike ufo-2 flowers, the flowers of 35S::LFYFLAG; 35S::UFO-myc; ufo-2 plants show a restoration of petals or petaloid organs and stamens owing to the activity of the transgenes (D,E).

  Weigel, D. and Nilsson, O. (1995). A developmental switch sufficient for flower initiation in diverse plants. Nature 377, 495-500.

  Supplemental Figure 2

• Supplemental Figure S2 -

  Fig. S2. Characterization of 35S::UFO-SRDX plants. (A-C) Scanning electron microscopy of 35S::UFO-SRDX plants. (A) A later arising flower with organs arranged in a whorl and fused carpels in the center. First whorl organs harbor stellate trichomes, which is a leaf-like characteristic. Number of organs reduced in the second and third whorls. (B) A whorled flower consisting of four sepal-like first organs with stellate trichomes, carpels in the center, and mosaic organs, such as carpelloid sepals and stamenoid sepals. (C) A lateral structure from the most severe lines (line 1 and 4 in Fig. S1D). The arrowheads indicate the stipules accompanying the leaf-like organs. There are internode elongations between those organs. The only floral tissues observed are papillae occasionally found at the tip of the leafy organs (arrow). (D) qRT-PCR analysis for UFO-SRDX transcript levels. Primer pairs were designed to distinguish UFO-SRDX transcripts from endogenous UFO transcripts. The Ct values are converted to relative values using either the 2-DDCt method or the DART-PCR program, which generated same pattern. Shown is the result from the DART-PCR program. UFOSRDX transcript levels are normalized to that of EF1a. Then, the relative values were calculated with the expression level of UFO-SRDX in wild type set as 1. The most severe lines (1 and 4) shown in Fig. 5G showed highest UFO-SRDX transcript levels. Other lines show general correlation between phenotypic severity and transgene expression levels.