DEV016550 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. The patterning and morphogenesis of vibrissae is severely impaired in the Catnb^Dex3K14/+mutant embryos. (A-D) Visualization of whisker placodes by Wnt10b and Sostdc1 expression in E12.75 and E13 embryos. Although the pattering of the original rows of vibrissae was not affected in the mutants, small extra placodes formed between and outside the rows and new placodes were initiated next to existing vibrissae placodes (arrows). In addition, extra sensory hairs formed in mutants (boxed areas). (E-H) Development of vibrissae of E12 control and E12 Catnb^Dex3K14/+mutant embryos in organ culture. After 3 days, the patterning of the mutant explant (F) was disturbed, the shapes of placodes were irregular and many placodes seemed to represent fusions of two placodes. After 2 more days in culture no vibrissae rows could be distinguished in the same explant and the buds had progressively more severe phenotypes. (I,J) Histological sections of E13.75 whisker pads. The control buds were uniformly spaced and sized, and the mesenchymal papillae were forming. In the Catnb^Dex3K14/+mutant the epithelium showed aberrant budding (arrow, J) and the layer of condensed mesenchyme was more pronounced that in the control. These features were similar to the developing hairs in mutant back skin (Figs 4I-L). Broken line indicates the epithelial-mesenchymal border. Scale bars: 1 mm in A; 200 µm in I.

• Supplemental Figure S2 -

  Fig. S2. Bmp4 expression in theCatnb^Dex3K14/+mutant skin. Additional vibratome sections of the same embryo as in Fig. 7K show that Bmp4 expression was present also in mesenchymal condensates in addition to the ectopic expression in the placode epithelium. Broken line indicates epithelium-mesenchyme border. Scale bar: 100 µm.