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Development ePress online publication date 11 Oct 2006
doi: 10.1242/dev.02620

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133/22/4475    most recent
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Research article

DNA supercoiling factor contributes to dosage compensation in Drosophila


Hirofumi Furuhashi, Mikage Nakajima, and Susumu Hirose*
* Author for correspondence (e-mail: shirose{at}lab.nig.ac.jp)

DNA supercoiling factor (SCF) is a protein capable of generating negative supercoils in DNA in conjunction with topoisomerase II. To clarify the biological functions of SCF, we introduced a heritable SCF RNAi into Drosophila. Upon knockdown of SCF, we observed male lethality and male-specific reduction in the expression levels of X-linked genes. SCF functionally interacts with components of the MSL complex, which are required for dosage compensation via hypertranscription of the male X chromosome. Moreover, SCF colocalizes with the MSL complex along the male X chromosome. Upon overexpression of SCF, the male X chromosome had a bloated appearance. This phenotype was dependent on the histone acetyltransferase MOF and was suppressed by simultaneous overexpression of ISWI. These findings demonstrate that SCF plays a role in transcriptional activation via alteration of chromatin structure and provide evidence that SCF contributes to dosage compensation.


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